​研究方向：

• 茶多酚之抗癌機制研究

• 以蛋白質體學方法系統性分析茶多酚作用蛋白

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過去與目前正在進行的研究計畫：​​​​

• 106年度：Trans 3,5,4'-trimethoxystilbene 增加雌激素抗性乳癌細胞對tamoxifen感受性之分子機制研究

• 105年度：槲皮素 (quercetin) 抑制三陰性乳癌細胞上皮細胞-間質細胞轉換與侵襲性表型之分子機制研究(3-3)

• 104年度：槲皮素 (quercetin) 抑制三陰性乳癌細胞上皮細胞-間質細胞轉換與侵襲性表型之分子機制研究(3-2)

• 103年度：槲皮素 (quercetin) 抑制三陰性乳癌細胞上皮細胞-間質細胞轉換與侵襲性表型之分子機制研究(3-1)

• 102年度：白藜蘆醇 (resveratrol) 與其甲氧基化結構衍生物抑制癌細胞上皮細胞-間質細胞轉換與侵襲性之分子機制研究(3-3)

• 101年度：白藜蘆醇 (resveratrol) 與其甲氧基化結構衍生物抑制癌細胞上皮細胞-間質細胞轉換與侵襲性之分子機制研究

• 100年度：白藜蘆醇 (resveratrol) 與其甲氧基化結構衍生物抑制癌細胞上皮細胞-間質細胞轉換與侵襲性之分子機制研究

• 99年度：紫檀氏(pterostilbene)抗發炎及抗腫瘤增生作用機制之探討(3/3)

• 98年度：紫檀氏抗發炎及抗腫瘤增生作用機制之探討(2/3)

• 97年度：紫檀氏抗發炎及抗腫瘤增生作用機制之探討(1/3)

• 96年度：以化學性蛋白質體學方法分析EGCG結合蛋白並評估其在化學癌症預防之重要性

• 95年度：以化學蛋白質體學方法分析茶紅素對蛋白酵素體抑制作用之選擇性

• 94年度：兒茶素EGCG抑制腫瘤相關性脂肪酸合成酵素機制之研究

• Jie-Heng Tsai, Li-Sung Hsu, Hsiu-Chen Huang, Chih-Li Lin, Min-Hsiung Pan, Hui-Mei Hong, and Wei-Jen Chen* (2016). 1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione induces G1 cell cycle arrest and autophagy in HeLa cervical cancer cells. International Journal of Molecular Sciences. Vol. 17, pp. 1274-88. doi:10.3390/ijms17081274

• Kuei-Yang Han, Maw-Soan Soon, Ming-Chian Hong, Chia-Cheng Kuo, Shih-Chan Lai, Ke-Ming Chen, Chih-Jung Chen, Kun-Tu Yeh, Li-Sung Hsu, Wei-Jen Chen* (2016). Naringenin attenuated prostate cancer invasion via reversed epithelial to mesenchymal transition and inhibited urokinase plasminogen activator activities. Experimental and Therapeutic Medicine. Vol. 17, pp. 1274-88. doi:10.3390/ijms17081274

• James Cheng-Chung Wei, Hsiu-Chen Huang, Wei-Jen Chen, Chien-Ning Huang, Chiung-Huei Peng, and Chih-Li Lin* (2016). Epigallocatechin gallate attenuates amyloid β-induced inflammation and neurotoxicity in EOC 13.31 microglia. European Journal of Pharmacology. Vol. 770, pp. 16-24.

• Wai-Fai Tung, Wei-Jen Chen, Hui-Chih Hung, Guang-Yaw Liu, Jai-Nien Tung, Chien-Chih Huang, and Chih-Li Lin* (2015). 4-Phenylbutyric Acid (4-PBA) and Lithium cooperatively attenuate cell death during oxygen-glucose deprivation (OGD) and reoxygenation. Cellular and Molecular Neurobiology. Vol. 35, pp. 849-859.

• Cheng Huang, Yi Jing Chen, Wei-Jen Chen, Chih-Li Lin, Yu Xuan Wei, and Hsiu Chen Huang* (2015). Combined treatment with Chrysin and 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose synergistically inhibits LRP6 and Skp2 activation in triple-negative breast cancer and xenografts. Molecular Carcinogenesis. Vol. 54, pp. 1613-1625.

• Tsai, Jie-Heng, Hsu, Li-Sung, Lin, Chih-Li, Hong, Hui-Mei, Pan, Min-Hsiung, Way, Tzong-Der, Chen, Wei-Jen* (2013). 3,5,4’-Trimethoxystilbene, a natural methoxylated analogue of resveratrol, inhibits breast cancer cell invasiveness by downregulation of PI3K/Akt and Wnt/β-catenin signaling cascades and reversal of epithelial-mesenchymal transition. Toxicology and Applied Pharmacology. Vol. 272, pp. 746-756.

• Tsai, M. L., Lai, C. S., Chang, Y. H., Chen, W. J., Ho, C. T. and Pan, M. H.* (2012). Pterostilbene, a natural analogue of resveratrol, potently inhibits 7,12-dimethylbenz[a]anthracene (DMBA)/12-Otetradecanoylphorbol-13-acetate (TPA)-induced mouse skin carcinogenesis. Food and Function. Vol. 3, pp. 1185-1194.

• Pan,M. H., Lin, C. L., Tsai, J. H., Ho, C. T. and Chen, W. J.* (2010). 3,5,3’,4’,5’-Pentamethoxystilbene (MR-5), a Synthetically Methoxylated Analogue of Resveratrol, Inhibits Growth and Induces G1 Cell Cycle Arrest of Human Breast Carcinoma MCF-7 Cells. Journal of Agricultural and Food Chemistry. Vol. 58, pp. 226-234.

• Lin, J. N., Lin, V. C., Rau, K. M., Shieh, P. C., Kuo, D. H., Shieh, J. C., Chen, W. J., Tsai, S. C. and Way, T. D.* (2010). Resveratrol Modulates Tumor Cell Proliferation and Protein Translation via SIRT1-Dependent AMPK Activation. Journal of Agricultural and Food Chemistry. Vol. 58, pp. 1584-1592.

• Pan, M. H., Lin, Y. T., Lin, C. L., Wei, C. S., Ho, C. T. and Chen, W. J.* (2009). Suppression of Heregulin-β1/HER2-Modulated Invasive and Aggressive Phenotype of Breast Carcinoma by Pterostilbene via Inhibition of Matrix Metalloproteinase-9, p38 Kinase Cascade and Akt Activation. Evidence-based Complementary and Alternative Medicine. Epub ahead of print: eCAM Advance Access published on Jul 16, 2009; doi:10.1093/ecam/nep093.

• Pan, M. H., Chiou, Y. S., Chen, W. J., Wang, J. M., Bad ma ev, V., Ho, C. T. (2009). Pterostilbene inhibited tumor invasion via suppressing multiple signal transduction pathways in hu ma n hepatocellular carcino ma cells. Carcinogenesis. Vol. 30, pp. 1234-1242 (SCI)

• Chen, W. J., Huang, Y. T., Wu, M. L., Huang, T. C., Ho, C. T. and Pan, M. H.* (2008). Induction of apoptosis by vitamin D2, ergocalciferol, via reactive oxygen species generation, glutathione depletion, and caspase activation in hu ma n leukemia Cells. Journal of Agriculture and Food Chemistry. Vol. 56, pp. 2996-3005 (SCI)

• Pan, M. H., Lin, C. C., Lin, J. K. and Chen, W. J.* (2007). Tea polyphenol EGCG suppresses heregulin-β1-induced fatty acid synthase expression in hu ma n breast cancer cells by inhibiting PI3K/Akt and MAPK cascade signaling. Journal of Agriculture and Food Chemistry. Vol. 55, pp. 5030-5037 (SCI)

• Chen, W. J. and Lin, J. K. (2004). Induction of G1 arrest and apoptosis in hu ma n Jurkat T cells by pentagalloylglucose through inhibiting proteasome activity and el eva ting p27Kip1, p21Cip1/WAF1 and Bax proteins. The Journal of Biological Chemistry, Vol. 279, pp. 13496-13505 (SCI)

• Chen, W. J. and Lin, J. K. (2004). Mechanisms of cancer chemoprevention by hop bitter acids (beer aro ma ) through induction of apoptosis mediated by Fas and caspase cascades. Journal of Agriculture and Food Chemistry, Vol. 52, pp. 55-64 (SCI)

• Chen, W. J., Chang, C. Y. and Lin, J. K. (2003). Induction of G1 phase arrest in MCF-7 hu ma n breast cancer cells by pentagalloylglucose through the down-regulation of CDK4 and CDK2 activities and up-regulation of the CDK inhibitors p27Kip and p21Cip. Biochemical Phar ma cology, Vol. 65, pp. 1777-1785 (SCI)

• Yeh, C. W., Chen, W. J., Chiang, C. T., Lin-Shiau, S. Y. and Lin, J. K. (2003). Suppression of fatty acid synthase in MCF-7 breast cancer cells by tea and tea polyphenols: a possible mechanism for their hypolipidemic effects. Phar ma cogenomics Journal, Vol. 3, pp. 267-276 (SCI)

• Ho, L. L., Chen, W. J., Lin-Shiau, S. Y. and Lin, J. K. (2002). Penta-O-galloyl-beta-D-glucose inhibits the invasion of mouse melano ma by suppressing metalloproteinase-9 through down-regulation of activator protein-1. European Journal of Phar ma cology, Vol. 23, pp. 1677-1684 (SCI)Pan, M. H., Chen, W. J., Lin-Shiau, S. Y., Ho, C. T. and Lin, J. K. (2002). Tangeretin induces cell-cycle G1 arrest through inhibiting cyclin-dependent kinases 2 and 4 activities as well as el eva ting Cdk inhibitors p21 and p27 in hu ma n colorectal carcino ma cells. Carcinogenesis, Vol. 453, pp. 149-158 (SCI)

• Wei, C. S. and Chen, W. J.* (2009). Effects of pterostilbene on heregulin-β1-associated signal transduction of hu ma n breast cancer cells. 第二十四屆生物醫學聯合學術年會.

• Tsai, J. H. and Chen, W. J.* (2009). Studies on the molecular mechanisms of 3,5,3',4',5'-pentamethoxystilbene induced G1 cell cycle arrest in MCF-7 hu ma n breast cancer cells. 第二十四屆生物醫學聯合學術年會.

• Lin, Y. T. and Chen, W. J.* (2008). Suppression of inducible nitric oxide synthase in lipopolysaccharides-activated ma crophages by pterostilbene. Sixteen symposium on recent advances in cellular and molecular biology. 第16屆細胞及分子生物新知研討會.

• Lin, C. C. and Chen, W. J.* (2007). Study on the mechanisms by which EGCG inhibits heregulin-β1-induced Fas expression. 第二十二屆生物醫學聯合學術年會.

• Chen, W. J.* (2006). Mechanisms by which (-)-epigallocatechin 3-gallate inhibits heregulin-beta1-induced fatty acid synthase expression in breast cancer cell lines. 232nd ACS National Meeting & Exposition.

• Lin, J. K., Chen, W. J. and Lin-Shiau, S. Y. (2005). Pentagalloylglucose induces G1 arrest and apoptosis through suppressing proteasome activity and el eva ting Cdk inhibitors and Bax proteins in hu ma n Jurkat T cells. Am eric an Association for Cancer research, 96th Annual Meeting.

• Chen, W. J. and Lin, J. K. (2005). Mechanisms by which (-)-epigallocatechin 3-gallate inhibits heregulin-β1-induced fatty acid synthase expression in breast cancer cell lines. Am eric an Association for Cancer research, 96th Annual Meeting.

• Chen, W. J. and Lin, J. K. (2003). Induction of G1 arrest in hu ma n Jurkat T cells by pentagalloylglucose through inhibiting proteasome activity and el eva ting p27Kip1 and p21Cip1/WAF1. 第十八屆生物醫學聯合學術年會.

• Chen, W. J. and Lin, J. K. (2002). Induction of apoptosis by α- ma ngostin in hu ma n leukemia HL-60 cells. 第十七屆生物醫學聯合學術年會.

• Pan, M. H. and Chen, W. J.* (2008) ACS Books "Functional Food and Health.": The cancer preventive potential of tea polyphenol EGCG in HER2-positive breast cancer. 2008 pp. 335-344

• Pan, M. H., Chen, W. J., Lo, C. H., Li, S., Sang, S., and Ho, C. H. (2008) ACS Books "Functional Food and Health.": Induction of apoptosis by acetylated black tea polyphenol through reactive oxygen species production, cytochrome c release, and caspase activation in hu ma n leukemia HL-60 cells. 2008 pp. 345-361